Recent and Selected Publications
Karwacki-Neisius, V+., Jang, A., Cukuroglu, E., Tai, A., Jiao, A., Predes, D., Yoon, J., Brookes, E., Chen, J., Iberg, A., Halbritter, F., Õunap, K., Gecz, J., Schlaeger, TM., Ho Sui, S., Göke, J., He, X., Lehtinen, MK., Pomeroy, SL., Shi, Y+. WNT signalling control by KDM5C during development affects cognition. Nature, 2024. +Co-correspondence.
Xu, WQ*., He, CX*., Kaye, EG., Li, JH*., Mu, MD., Nelson, GM., Dong, L., Wang, JH., Wu, FZ., Shi, YJG., Adelman, K+., Lan, F+., Shi, Y+., and Shen, HJ+. Dynamic control of chromatin associated m6A methylation regulates nascent RNA synthesis. Mol Cell, 2022 Mar; 17;82(6):1156-1168.e7. Epub 2022 Feb 25. *Equal contributions; +Co-correspondence. PDF
Liu, Y., Debo, B., Li, MF, Shi, ZN, Sheng, WQ+., and Shi, Y+. LSD1 inhibition sustains T cell invigoration with a durable response to PD-1 blockade. Nature Commun., 2021 Nov 24; 12(6831). +Co-correspondence. PDF
Blanco, AM*+., Skyes, DB*., Gu, L*., Wu, MJ., Petroni, R., Karnik, R., Wawer, M., Rico, J., Li, HT., Jacobus, WD., Jambhekar, A., Cheloufi, S., Meissner, A., Hochedlinger, K., Scadden, DT+., and Shi, Y+. Chromatin state barriers enforce an irreversible mammalian cell fate decision. Cell Rep. 2021 Nov 9; 37(6):109-967. *Equal contributions; +Co-correspondence. PDF
Xu, WQ., Li, JH., He, CX., Wen, J., Ma, HH., Rong, BW., Dian, JB., Wang, LY., Wang, JH., Wu, FZ., Tan, L., Shi, YG., Shi, Y+., and Shen, HJ+. METTL3 regulates heterochromatin in mouse embryonic stem cells. Nature, 2021 Mar; 591(7849):317-321, Epub 2021 Jan 27. +Co-correspondence. PDF
Sheng, WQ*., Liu, Y*., Chakraborty, D., Debo, B., and Shi, Y. Simultaneous inhibition of LSD1 and TGF-b enables eradication of poorly immunogenic tumors with anti-PD-1 treatment. Cancer Discovery, 2021 Mar;11(8):1970-1981. *Equal contribution. PDF
Chen, H*., Liu, Q*., Yu, D., Natchiar, K., Zhou, C., Hsu, CH., Hsu, PH., Zhang, X., Klaholz, B., Gregory, RI., Cheng, XD, and Shi, Y. METTL5, an 18S rRNA-specific m6A methyltransferase, regulates the integrated stress response. bioRxiv 2020.04.27.064162. *Equal contribution. PDF
Chen, H., Gu, L., Orellana, EA., Wang, Y., Guo, J., Liu, Q., Wang, L., Shen, Z., Wu, H., Gregory, RI., Xing, Y., Shi, Y. METTL4 is an snRNA m6Am methyltransferase that regulates RNA splicing. Cell Res. 2020 Jan 8; 30(6):544-547. PDF
Anastas, JN., Zee, BM., Kalin, JH., Kim, MH., Guo, R., Alexandrescu, S., Blanco, MA., Giera, S., Gillespie, SM., Das, J., Wu, MH., Nocco, S., Bonal, D.M., Suva, M.L., Bernstein, BE., Alani, R., Golub, TR., Cole, PA., Filbin, MG+., and Shi, Y+. Re-programming chromatin with a bifunctional LSD1/HDAC inhibitor induces therapeutic differentiation in DIPG. Cancer Cell, 2019; 36(5):528-544. +Co-correspondence. PDF
Sendinc, E*, Valle-Garcia, D*., Dhall, A., Chen, H., Henriques, T., Navarrete-Perea, J., Sheng, WQ., Gygi, SP., Adelman, K., Shi, Y. PCIF1 catalyzes m6Am methylation to regulate gene expression. Mol Cell. 2019; 5(3):620-630.e9. Epub 2019 Jul 3. *Equal contribution. PDF
Sheng, W.Q.*, LaFleur, M.W.*, Nguyen, T.H., Chen, S.J., Chakravarthy, A., Conway, J.R., Li, Y., Chen, ., Yang, H., Hsu, P-H., Van Allen, E.M., Freeman, G.J., De Carvalho, D.D., He, H.H.H., Sharpe, A.H.+, and Y. Shi+. LSD1 ablation in tumor cells simulates anti-tumor immunity and enables checkpoint blockade. Cell, 2018 Jul 26; 174(3):549-563, Online publication 06/21/2018. *Equal contribution; +Co-correspondence. PDF
Xiang, Y*., Laurent, B*., Hsu, C-H*., Nachtergaele, S., Lu, ZK., Xu, CY., Sheng, WQ., Jian, OY., Wang, SQ., Ling, D., Hsu, P-H., Zou, L., Jambhekar, A., He, C., and Shi, Y. m6A RNA methylation regulates the UV-induced DNA damage response. Nature, 2017 Mar 23; Vol 543:573-576. *Equal contribution. PDF
Li HJ*, Liefke R*, Jiang JY, Tian W, Deng PJ, Zhang WD, He Q, Patel DJ, Shi Y, Wang ZX. Polycomb-like proteins link the PRC2 complex to CpG islands. Nature, 2017 Sep 14; 549(7671):287-291. * Equal contributions. Bold indicates members of the Shi lab.
Liefke R, Karwacki-Neisius V, Shi Y. EPOP interacts with Elongin BC and Usp7 to modulate the chromatin landscape. Mol Cell. 2016 Nov 17; 64(4):659-672. PDF
Shen, HJ*., Xu, WQ*., Guo, R., Rong, BW., Gu, L., Wang, ZT., He, CX., Zheng, LJ., Hu, X., Shao, ZM., Yang, PY., Wu, FZ., Shi, YJG, Shi, Y+., and Lan, F+. Suppression of enhancer over-activation by a RACK7-histone demethylase complex. Cell, 2016 Apr 7; Vol 165(2):331-42. *Equal contribution, +Co-correspondence. PDF
Iwase S*, +, Brookes E*, Agarwal S*, Badeaux AI, Ito H, Tomassy GS, Kasza T, Vallianatos C, Lin G, Thompson A, Gu L, Kwan KY, Chen CF, Sarto MA, Egan B, Xu J+, Shi Y+. A mouse model of X-linked intellectual disability associated with impaired removal of histone methylation. Cell Rep. 2016 Feb 9; 14(5):1000-1009. *Equal contribution, +Co-correspondence. PDF
Greer EL+, Becker B, Latza C, Antebi A, Shi Y+. Mutation of C. elegans demethylase spr-5 extends trans-generational longevity. Cell Res. 2016 Feb; 26(2):229-238. +Co-correspondence. PDF
Greer EL* +, Blanco MA*, Gu L, Sendinc E, Liu JZ, Aristizábal-Corrales D, Hsu CH, Aravind L, He C, Shi Y +. DNA methylation on N6-adenine in C. elegans. Cell, 2015 May 7; 161(4): 868-878 (Cover). * Equal contributions, +Co-correspondence. PDF
Murn J+, Zarnack K, Yang YJ, Durak O, Murphy EA, Cheloufi S, Gonzalez DM, Teplova M, Curk T, Zuber J, Patel DJ, Ule J, Luscombe NM, Tsai LH, Christopher A, Shi Y+. Control of a neuronal morphology program by an RNA-binding zinc finger protein, Unkempt. Genes & Dev. 2015 Mar 1; 29(5):501-512 (Cover). +Co-corresponding PDF
Laurent B, Ruitu L, Murn J, Hempel K, Ferrao R, Xiang Y, Liu S, Garcia BA, Wu H, Wu F, Steen H, Shi Y. A Specific LSD1/KDM1A isoform regulates neuronal differentiation through H3K9 demethylation. Mol Cell. 2015 Mar 19; 57(6): 957-970. Preview: Shin JH, Ming GL, Song HJ. Molecular toggle switch of histone demethylase LSD1. Mol Cell. 2015 Mar 19; 57(6):949-950. PDF
Alpatov R*, Lesch BJ*, Nakamoto-Kinoshita M, Blanco A, Chen S, Stützer A, Armache KJ, Simon MD, Xu C, Ali M, Murn J, Prisic S, Kutateladze TG, Vakoc CR, Min J, Kingston RE, Fischle W,Warren ST, Page DC and Shi Y. A Chromatin-Dependent Role of the Fragile X Mental Retardation Protein FMRP in the DNA Damage Response. Cell, 2014 May 8; 157(4):869-81. *Equal contribution. PDF
Greer EL, Beese-Sims S, Brookes E, Spadafora R, Zhu Y, Rothbart SB, Aristizabal-Corrales D, Chen SZ, Badeaux AI, Qin QY, Wang W, Strahl BD, Colaiacovo MP, Shi Y. A histone methylation network regulates transgenerational epigenetic memory in C. elegans. Cell Rep. 2014 Apr 10; 7(1):113-126. PDF
Mosammaparast N+, Kim H, Laurent B, Dango S, Luo Y, Sowa M, Lim HJ, Majid M, Gygi S, Harper W, Yankner B, Shi Y+. The histone demethylase LSD1/KDM1A promotes the DNA damage response. J. Cell. Biol. 2013 Nov 11; 203(3): 457-470. +Co-correspondence. PDF
Chen S, Ma J, Wu F, Xiong LJ, Villen J, Gygi SP, Liu XS, Shi Y. The histone H3 Lys 27 demethylase JMJD3 regulates gene expression by impacting transcription elongation. Genes & Dev. 2012 Jun 15; 26(12):1364-1375. PDF
Dango S*, Mosammaparast N*, Sowa ME, Xiong LJ, Wu F, Park K, Rubin M, Gygi SP, Harper JW, Shi Y. DNA unwinding by ASCC3 helicase is coupled to ALKBH3-dependent DNA alkylation repair and cancer cell proliferation. Mol Cell. 2011 Nov 4; 44(3):373-384. *Equal contribution. PDF
Greer EL, Maures TJ, Ucar D, Hauswirth AG, Mancini E, Lim JP, Benayoun BA, Shi Y, Brunet A. Transgenerational epigenetic inheritance of longevity in Caenorhabditis elegans. Nature, 2011 Oct 19; 479(7373):365-371. PDF
Iwase S, Xiang B, Ghosh S, Ren T, Lewis PW, Cochrane JC, Allis CD, Picketts DJ, Patel DJ+, Li H+, Shi Y+. ATRX ADD domain links an atypical histone methylation recognition mechanism to human mental-retardation syndrome. Nat Struct Mol Biol. 2011 Jun 12; 18(7):769-776. +Co-correspondence. PDF
Fang R, Barbera A, Ruternberg M, Lian C, Leonor T, Lan F, Shimizu F, Sui GC, Shi Y, Shi YJG. Human LSD2/KDM1b/AOF1 regulates gene transcription by modulating intragenic H3K4me2 methylation. Mol Cell. 2010 Jul 30; 39(2):222-233. PDF
Qi, HH*., Sarkissian, M*., Hu, G.Q., Zhao, Z.B., Bhattacharjee, A., Gordon, B., Lim, S., Lan, F., Yaghi, NK., Garcia B., Brizuela, L., Zhao, KJ., Roberts, TM*, and Shi, Y*. Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development. Nature, 2010 Jul 22; 446:503-07. *Equal contribution, +Co-correspondence. PDF
Horton JR*, Upadhyay AK*, Qi HH*, Zhang X, Shi Y, Cheng XD. Enzymatic and structural insights for substrate specificity of a family of jumonji histone lysine demethylases. Nat Struct Mol Biol. 2010 Jan; 17(1):38-43. *Equal contribution. PDF
Chen SZ, Whetstine J, Ghosh S, Hanover JA, Shi Y. The conserved NAD(H)-dependent corepressor CTBP-1 regulates Caenorhabditis elegans life span. Proc Natl Acad Sci USA. 2009 Feb 3; 106(5):1496-1501. PDF
Mulligan P, Westbrook T, Ottinger M, Macia E, Liu JS, Shi YJ, Barretina J, Howley P, Elledge S, Shi, Y. CDYL bridges REST and histone methyltransferases for gene repression and suppression of cellular transformation. Mol Cell. 2008 Dec 5; 32(5):718-726 (Featured Article, highlighted by Cell. 2008; 135 Leading Edge pg. 981). PDF
Lan, F., Bayliss, PE., Rinn, J., Whetstine JR., Wang JK., Chen, SZ., Iwase, S., Alpatov, R., Issaeva, I., Canaani, E., Roberts, TM., Chang, HY., and Shi, Y. A histone H3 lysine 27 demethylase regulates animal posterior development. Nature, 2007 Oct 11; 449:689-94. (Mini-review Cell 2007 Vol 131: 29-32). PDF
Lan, F*., Collins, R*., De Cegli, R., Alpatov, R., Horton, J.R., Shi, XB, Gozani, R., Cheng, XD+, and Shi, Y+. Recognition of unmethylated H3K4 links BHC80 to LSD1 mediated gene repression. Nature, 2007 Aug 9; 448:718-23. *Equal contribution, *Co-correspondence. PDF
Liu HF, Schmidt-Supprian M, Shi YJ, Hobeika E, Barteneva N, Jumaa H, Pelanda R, Reth M, Skok J, Rajewsky K, Shi, Y. Yin Yang 1 is a critical regulator of B-cell development. Genes & Dev. 2007 May 15; 21(10):1179-1189. (Perspective by Calame K and Atchison M. YY1 helps to bring loose ends together. Genes & Dev. 2007 May 15; 21(10):1145-1152). PDF
Lan F, Zaratiegui M, Villen J, Vaughn M, Verdel A, Gygi S, Moazed D, Martienssen R, Shi Y. S. pombe LSD1 homologs regulate heterochromatin propagation and euchromatin gene transcription. Mol Cell, 2007 Apr 13; 26(1):89-101. (Preview: Chosed R, Dent SY. A two way street: LSD1 regulates chromatin boundary formation in S. pombe and Drosophila. Mol Cell. 2007 Apr 27; 26(2):160-162). PDF
Iwase, S, Lan, F., Bayliss, P., de la Torre-Ubieta, L., Qi, H., Huarte, M., Whetstine, JR.,Bonni, A., Roberts, T., and Shi, Y. The X-linked mental retardation gene SMCX/JARID1C defines a family of histone H3 lysine 4 demethylases. Cell, 2007 Mar 23; 128:1077-88. PDF
Chen Z, Zhang J, Whetstine JR, Hong X, Davrazou F, Kutateladze TG, Simpson M, Mao Q, Pan CH, Dai S, Hagman J, Hansen K, Shi Y, Zhang G. Structural insights into histone demethylation by JMJD2 family members. Cell 2006 May 19; 125(4):691-702. PDF
Whetstine, JR., Nottke, A., Lan, F., Huarte, M., Smolikov, S., Chen, Z., Spooner, E., Li, E., Zhang, G., Colaiacovo, M., and Shi, Y. Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases. Cell, 2006 May 5; 125:467-481. [Commented in Research Highlight, Nat. Rev. Mol. Cell. Biol. June, 2006]. PDF
Shi YJ, Matson C, Lan F, Iwase S, Baba T, Shi Y. Regulation of LSD1 histone demethylase activity by its associated factors. Mol Cell. 2005 Sep 16; 19(6):857-864. (Minireview in Cell 2005; 122:654-658). PDF
Shi, Y.J., Lan, F., Matson, C., Mulligan, P., Whetstine, J.R., Cole, P.A., Casero, R.A. and Shi, Y. Histone demethylation mediated by the nuclear amine oxidase homolog LSD1. Cell, 2004 Dec 29; Vol 119:941-953. [Mini-review in Cell 119:903-906, findings highlighted in Science 306:2171 (2004), Nature 433:29 (2005), Nature Reviews Mol. Cell Biol. (2005), Faculty of 1000 All Time Top 10 in Biology, one of the 25 Landmark publications of 40 years of Cell. PDF
Sui GC, Affar EB, Shi YJ, Wall N, Brignone C, Yin P, Dohonoe M, Calvo D, Grossman S, Shi Y. Yin Yang 1 is a negative regulator of p53. Cell, 2004 Jun 25; 117(7):859-872. PDF
Shi, Y. J., Sawada, J-I., Sui, G.C., Affar, E.B., Whetstine, J., Lan, F., Ogawa, H., Luke, M., P-S., Nakatani, Y., and Shi, Y. Coordinated histone modifications mediated by a CtBP co-repressor complex. Nature, 2003 Apr 17; 422:735-8. PDF
Gay F, Calvo D, Lo MC, Ceron J, Maduro M, Lin RL, Shi Y. Acetylation regulates subcellular localization of the Wnt signaling nuclear effector POP-1. Genes & Dev. 2003 Mar 15; 17(6):717-22. PDF
Sui, G.C., Soohoo, C, Affar, E.B., Shi, Y.J., Forrester, W., and Shi, Y. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc. Natl. Acad. Sci. USA. 2002 Apr 16; 99:5515-20. PDF
Shi Y*, Mello C. A CBP/p300 homolog specifies multiple differentiation pathways in caenorhabditis elegans. Genes & Dev. 1998 Apr 1; 12(7):943-955. (Cover Photo). Commented in: Trends in Genetics 14:183,1998; Nature Biotechnology 16:405, 1998. *Co-correspondence. PDF
Lee JS, Galvin KM, See RH, Eckner R, Livingston D, Moran E, Shi Y. Relief of YY1 transcriptional repression by adenovirus E1A is mediated by E1A-associated protein p300. Genes & Dev.1995 May 15; 9(10):1188-1198. PDF
Selected Review Articles
Sendinc, E., Shi, Y. (2023). RNA m6A methylation across the transcriptome. Mol Cell. Vol 83(3): 428-41. PDF
Jambhekar, A., Dhall, and Shi, Y. (2019). Roles and regulation of histone methylation in animal development. Nat Rev Mol Cell Biol. Vol 20(10):625-641. PDF
Murn, J., and Shi, Y. (2017). The winding path of protein methylation research: milestones and new frontiers. Nat Rev Mol Cell Biol. Vol 18(8):517-527. PDF
Jambhekar, A., Anastas, JN., and Shi, Y. (2017). Histone lysine demethylase inhibitors. Cold Spring Harb Perspect Med. Vol 7(1). PDF
Brookes, E. and Shi, Y. (2014). Disparate epigenetic mechanisms of human disease, Ann Rev Genet. Vol 48:237-68. PDF
Badeaux AI, Shi Y. (2013). Emerging roles for chromatin as a signal integration and storage platform. Nature Rev Mol Cell Biol. Vol 14(4): 211-24. PDF
Greer EL, Shi Y. (2012). Dynamic regulation of histone methylation in health, disease, and inheritance. Nat Rev Genet. Vol 13(5):343-357. PDF
Mosammaparast N, Shi Y. (2010). Reversal of histone methylation: Biochemical and molecular mechanisms of histone demethylases. Annu Rev Biochem. Vol 79:155-79. PDF
Lan F, Shi Y. (2009). Epigenetic regulation: methylation of histones and non-histone proteins. Sci China C Life Sci. Vol 52(4):311-22. PDF
Nottke AC, Colaiacovo M, Shi Y. (2009). Developmental roles for the histone lysine demethylases. Development. Vol 136(6):879-89. PDF
Shi Y. (2007). Histone demethylases: emerging roles in development, physiology and disease. Nat Rev Genet. Vol 8(11):829-33. PDF
Shi Y*, Whetstine J. (2007). Dynamic regulation of histone lysine methylation by demethylases. Mol Cell. Vol 25(1):1-14. *Correspondence. PDF
Shi YJ, Shi Y. (2004). Metabolic enzymes in transcription-a direct link between metabolism and regulated gene expression? Trends in Genetics. Vol 20:445-52. PDF
Shi Y. (2003). Mammalian RNAi for the masses. Trends in Genetics. Vol 19(1):9-12 (#1 downloaded article among all of Elsevier publications in 2003). PDF
Shi Y, Lee JS, Galvin KM. (1997). Everything you have ever wanted to know about Yin Yang 1…. Biochim Biophys Acta. 1332(2): F49-66. PDF